Category: 69205-79-4

Ghosh, Kuntal; Choi, Youbin; Lee, Jae Won; Baraki, Haftom; Kim, Kwang-Pyo published the artcile< Complete nucleotide sequence analysis and identification of 7-cyano-7-deazaguanine biosynthesis-related genes in the novel Bacillus subtilis-infecting Siphoviridae family phage BSP7>, Synthetic Route of 69205-79-4, the main research area is sequence cyano deazaguanine bacteriophage Bacillus Siphoviridae.

In this study, bacteriophage BSP7, a novel Bacillus subtilis-infecting member of the family Siphoviridae, was isolated from a Korean soybean-based fermented food, Deonjang, using B. subtilis ATCC 21336 as a host. The genome is 55,455 bp long with 39.92% G+C content. A total of 70 ORFs with no tRNA were detected in the genome. A distinct feature of the BSP7 genome among B. subtilis-infecting Siphoviridae family phages is the presence of putative ORFs related to biosynthesis of 7-cyano-7-deazaguanine (PreQ0), a precursor of queuosine and archaeosine biosynthesis. Bioinformatic anal. revealed that the genome of BSP7 does not exhibit any significant similarities to other phages with sequences in the NCBI database. A comparative genomic anal. also confirmed the uniqueness of BSP7 within the family Siphoviridae. The whole genome sequence of Bacillus subtilis-infecting phage BSP7 has been deposited in GenBank database under accession number MH707430.

Archives of Virology published new progress about Antibiotic resistance. 69205-79-4 belongs to class nitriles-buliding-blocks, and the molecular formula is C7H5N5O, Synthetic Route of 69205-79-4.

Referemce:
Nitrile – Wikipedia,
Nitriles – Chemistry LibreTexts

Jung, Jihye; Braun, Jan; Czabany, Tibor; Nidetzky, Bernd published the artcile< Interplay of nucleophilic catalysis with proton transfer in the nitrile reductase QueF from Escherichia coli>, Related Products of 69205-79-4, the main research area is interplay nucleophilic catalysis proton transfer nitrile reductase QueF Escherichia.

Enzymic transformations of the nitrile group are important in biol. as well as in synthetic chem. The enzyme QueF catalyzes the conversion of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1), a unique approach towards biol. four-electron reduction of a nitrile to an amine. The catalytic reaction involves a QueF-preQ0 thioimidate adduct that is converted to preQ1 in two NADPH dependent reduction steps via an imine intermediate. The QueF active site comprises a cysteine nucleophile flanked by an aspartic acid and addnl. contains a histidine. Here, we used mutagenesis of E. coli QueF (C190A, C190S, D197A, D197H, and H229A) to study the functional interplay between these enzyme residues in covalent catalysis. Substitution of Cys190 or Asp197 annihilates preQ0 covalent binding and largely disrupts the nitrile-to-amine reductase activity. The H229A variant readily forms the thioimidate adduct and is 24-fold less active for preQ0 reduction than wild-type ecQueF (kcat = 7.2 min-1). Using isothermal titration calorimetry, we show that the non-covalent step of preQ0 binding involves proton uptake mediated by Asp197 with His229 as the likely protonated group. Catalytic proton transfer from the Cys190 thiol via Asp197 to the nitrile nitrogen promotes the covalent intermediate. We suggest that protonated (charged) His229 facilitates the polarization of the substrate nitrile for nucleophilic attack on carbon by Cys190, and through proton relay via Asp197, it could provide the proton for re-protonating Cys190 during the formation of the imine intermediate.

Catalysis Science & Technology published new progress about Enzyme functional sites, active. 69205-79-4 belongs to class nitriles-buliding-blocks, and the molecular formula is C7H5N5O, Related Products of 69205-79-4.

Referemce:
Nitrile – Wikipedia,
Nitriles – Chemistry LibreTexts