《Design, synthesis, biological evaluation and molecular docking of new uracil analogs-1,2,4-oxadiazole hybrids as potential anticancer agents》 was written by El Mansouri, Az-Eddine; Oubella, Ali; Maatallah, Mohamed; AitItto, Moulay Youssef; Zahouily, Mohamed; Morjani, Hamid; Lazrek, Hassan B.. Recommanded Product: 4-Cyanobenzyl bromideThis research focused onuracil oxadiazole hybrid preparation caspase mol docking anticancer agent; 1,2,4-Oxadiazole; Anticancer activity; Apoptosis; Heterogeneous catalyst; Hybrid molecules; Molecular docking; Uracil analogues. The article conveys some information:
A new series of uracil analogs-1,2,4-oxadiazole hybrid derivatives were synthesized by a new, simple, and efficient method using for the first time HAP-SO3H as an heterogeneous acid catalyst for the condensation and cyclization between amidoxime and aldehyde. The new derivatives were characterized by HRMS, FTIR, 1H NMR, and 13C NMR spectroscopy techniques. The synthesized 1,2,4-oxadiazole hybrids were evaluated for their cytotoxic activity in five human cancer cell lines: melanoma (A-375), fibrosarcoma (HT-1080), breast (MCF-7 and MDA-MB-231), and lung carcinoma (A-549). Compounds 1-[4-(5-Biphenyl-4-yl-[1,2,4]oxadiazol-3-yl)-benzyl]-5-fluoro-1H-pyrimidine-2,4-dione and 5-Fluoro-1-{4-[5-(4′-methoxy-biphenyl-4-yl)-[1,2,4]oxadiazol-3-yl]-benzyl}-1H-pyrimidine-2,4-dione were potent cytotoxic agents against HT-1080 and MFC-7 cells with IC50 inferior to 1μM. The possible mechanism of apoptosis induction by the derivatives was studied using Annexin v staining, caspase-3/7 activity, mitochondrial membrane potential measurement, and anal. cell cycle progression. The compound 1-[4-(5-Biphenyl-4-yl-[1,2,4]oxadiazol-3-yl)-benzyl]-5-fluoro-1H-pyrimidine-2,4-dione induced apoptosis through caspase-3/7 activation and S-phase arrest in HT-1080 and A549 cells. The mol. docking showed that compound 1-[4-(5-Biphenyl-4-yl-[1,2,4]oxadiazol-3-yl)-benzyl]-5-fluoro-1H-pyrimidine-2,4-dione activated the caspase-3 by forming a stable protein-ligand complex. In the experiment, the researchers used many compounds, for example, 4-Cyanobenzyl bromide(cas: 17201-43-3Recommanded Product: 4-Cyanobenzyl bromide)
4-Cyanobenzyl bromide(cas: 17201-43-3) can reacts with 2H-tetrazole in the presence of KOH to yield 4-[(2H-tetra-zol-2-yl)methyl]benzonitrile. And it may be used in the synthesis of ligands containing a chelating pyrazolyl-pyridine group with a pendant aromatic nitrile.Recommanded Product: 4-Cyanobenzyl bromide
Referemce:
Nitrile – Wikipedia,
Nitriles – Chemistry LibreTexts