The preparation of ester heterocycles mostly uses heteroatoms as nucleophilic sites, which are achieved by intramolecular substitution or addition reactions. Compound: 5-Chloro-1-methyl-4-nitroimidazole( cas:4897-25-0 ) is researched.Recommanded Product: 5-Chloro-1-methyl-4-nitroimidazole.Zhuang, Jian-fen; Zeng, Huan-qun published the article 《Determination of related substance in azathioprine by HPLC》 about this compound( cas:4897-25-0 ) in Zhongguo Xiandai Yingyong Yaoxue. Keywords: HPLC azathioprine mercaptopurine chloro methyl nitroimidazole. Let’s learn more about this compound (cas:4897-25-0).
The objective of this paper is to establish an HPLC method for the determination of related substance in azathioprine. Phenomenex C18 (4.6 mm × 200 mm, 5 μm) column was used, the mobile phase consisted of methanol-0.05% sodium acetate (18:82), at flow rate of 1.2 mL/min-1, detection wavelength was set at 240 nm. The results show that on the selection chromatog. condition, related substances of azathioprine, starting material, intermediate and etc were separated completely. The linear ranges were 0.45-1.125 μg/mL-1 (r = 0.9999) for mercaptopurine and 0.3-0.75 μg/mL-1 (r = 0.9999) for 5-chloro-1-methyl-4-nitroimidazole. The average recoveries (n = 9) of mercaptopurine and 5-chloro-1-methyl-4-nitroimidazole were 100.1% and 99.7% resp. The limit of quantitations were 3 ng/mL-1 and 2 ng/mL-1. It was concluded that the method proposed for the determination of the related substances of azathioprine is simple, accurate, and specific.
In addition to the literature in the link below, there is a lot of literature about this compound(5-Chloro-1-methyl-4-nitroimidazole)Recommanded Product: 5-Chloro-1-methyl-4-nitroimidazole, illustrating the importance and wide applicability of this compound(4897-25-0).
Reference:
Nitrile – Wikipedia,
Nitriles – Chemistry LibreTexts