Chemistry is an experimental science, Application In Synthesis of 4-Chlorobenzonitrile, and the best way to enjoy it and learn about it is performing experiments.Introducing a new discovery about 623-03-0, Name is 4-Chlorobenzonitrile, molecular formula is C7H4ClN, belongs to nitriles-buliding-blocks compound. In a document, author is Zou, Shuping.
A integrated process for nitrilase-catalyzed asymmetric hydrolysis and easy biocatalyst recycling by introducing biocompatible biphasic system
The whole-cell nitrilase-catalyzed asymmetric hydrolysis of nitriles is a green and efficient preparation approach for chiral carboxylic acids, but often suffers from toxicity and cell lysis from organic substrates. In this work, a novel integrated process for whole-cell nitrilase-catalyzed asymmetric hydrolysis was developed for the first time by introducing a biocompatible ionic liquid (IL)-based biphasic system. The whole-cell nitrilases displayed an outstanding stability and recyclability in the biphasic system and still retained > 85% activity even after 7 cycles reaction. A preparative-scale fed-batch hydrolysis of o-chloromandelonitrile to (R)-o-chloromandelic acid (RCMA) was performed using the integrated process. The results revealed a yield of 91.3% and a space-time yield of 746.4 g.L-1.d(-1), which are currently the highest reported values for R-CMA biosynthesis. The proposed integrated process avoids substrate inhibition, facilitates the reusability of whole-cell nitrilases, and thus shows great potential for the sustainable production of chiral carboxylic acids.
Sometimes chemists are able to propose two or more mechanisms that are consistent with the available data. If a proposed mechanism predicts the wrong experimental rate law, however, the mechanism must be incorrect.Welcome to check out more blogs about 623-03-0, in my other articles. Application In Synthesis of 4-Chlorobenzonitrile.