Nitration of m-tolunitrile and identification of the products was written by Shi, Zhi-hao;Wei, Yun-yang;Qin, Tao;Mu, Fei-hu. And the article was included in Jingxi Huagong in 2005.HPLC of Formula: 64113-86-6 This article mentions the following:
Three nitro isomers of m-tolunitrile were obtained in 92% total yield via nitration of m-tolunitrile with concentrated nitric acid (≥98%) under the following conditions: n(m-tolunitrile):n(nitric acid) = 1:10, reaction time 2 h, reaction temperature 18 ∼ 20 °C and dropping nitric acid to m-tolunitrile in ice bath. Column chromatog. followed by recrystallization gave three pure isomers 5-cyano-2-nitrotoluene, 3-cyano-4-nitrotoluene and 3-cyano-2-nitrotoluene in 43%, 37% and 12% yield resp., based on m-tolunitrile. Structure of the pure isomers was characterized by IR, 1HNMR and GS/MS. Regioselectivity of the nitration was discussed. In the experiment, the researchers used many compounds, for example, 5-Methyl-2-nitrobenzonitrile (cas: 64113-86-6HPLC of Formula: 64113-86-6).
5-Methyl-2-nitrobenzonitrile (cas: 64113-86-6) belongs to nitriles. There has been no report on the microbial biosynthesis of nitriles and the physiological function of such enzymes, nor was it not even known whether aliphatic and aromatic nitriles are biological compounds or just petrochemicals. Industrially, the main methods for producing nitriles are ammoxidation and hydrocyanation. Both routes are green in the sense that they do not generate stoichiometric amounts of salts.HPLC of Formula: 64113-86-6
Referemce:
Nitrile – Wikipedia,
Nitriles – Chemistry LibreTexts