Mulder, A. H. published the artcileInhibitory action of 2,6-dichloro-3-hydroxybenzonitrile and 2,6-dichloro-4-hydroxybenzonitrile on the beating of heart cells in tissue culture. Antagonism of oligomycin, Synthetic Route of 3336-34-3, the publication is Biochimica et Biophysica Acta, Enzymology and Biological Oxidation (1966), 128(2), 391-3, database is CAplus.
The effects of 2,6-dichloro-3-hydroxybenzonitrile (I) and 2,6-dichloro-4-hydroxy-benzonitrile (II), uncouplers of oxidative phosphorylation, on the beating of heart cells were studied and compared with those obtained with 2,4-dinitrophenol (III). Concentrations of I, II, and III necessary to arrest beating were 0.3, 0.24, and 0.41 mM, resp. Oligomycin, when added 5 min. after these phenols had arrested the beating of the cells, partially restored the beating of I- or III-arrested cells, and completely restored the beating of II-arrested cells. ATP, to varying degrees, restored the beating of the phenol-arrested cells. III-treated cells started beating within 60 sec. after ATP addition, whereas I-treated cells began to beat 2-3 min. after ATP addition Cultures pretreated with II did not respond to ATP during the time of observation (6 min.). When II-inhibited cells were treated with oligomycin (20 γ/ml.) 6 min. after ATP addition, they started to beat again. A rise in frequency by ATP addition after oligomycin treatment was also observed on I-and III-inhibited cells. The differences in response to ATP additions by cells pretreated with these phenols probably reflect the quantitative differences in induction of ATPase in the living cells. The antagonism of oligomycin to the 2,6-dichlorobenzonitriles is in good agreement with the previous conclusion that the compounds act on the mitochondria and not on the cytoplasmic constituents of the living cell.
Biochimica et Biophysica Acta, Enzymology and Biological Oxidation published new progress about 3336-34-3. 3336-34-3 belongs to nitriles-buliding-blocks, auxiliary class Chloride,Nitrile,Benzene,Phenol, name is 2,6-Dichloro-3-hydroxybenzonitrile, and the molecular formula is C7H3Cl2NO, Synthetic Route of 3336-34-3.
Referemce:
https://en.wikipedia.org/wiki/Nitrile,
Nitriles – Chemistry LibreTexts