Xu, Hong-mei published the artcilePreparation of (R)-o-chloromandelic acid through dynamic kinetic resolution of o-chloromandelonitrile by whole cells of Alcaligenes faecalis CGMCC 1.2006, Quality Control of 13312-84-0, the publication is Fenzi Cuihua (2014), 28(2), 174-181, database is CAplus.
Alcaligenes faecalis CGMCC 1.2006 with higher arylacetonitrilase activity and moderate enantioselectivity towards o-chloromandelonitrile was screened from six microbial strains belonging to Alcaligenes faecalis. The regulating effects of reaction medium on both activity and enantioselectivity of arylacetonitrilase were studied. The addition of water-soluble co-solvents such as methanol into the reaction medium decreased the activity of arylacetonitrilase, but enhanced the enantioselectivity significantly. Addition of non-ionic surfactants possessed the reverse effects. The effects of reaction conditions such as substrate concentration, cell concentration, pH, temperature and reaction time on the activity and enantioselectivity of arylacetonitrilase were also optimized systematically. Under the optimal reaction conditions, the fed-batch production of (R)-o-chloromandelic acid was attained with 32.2 g/L productivity within 22 h, in 82.4% yield and 93.1% ee. With o-chlorobenzaldehyde and potassium cyanide as the substrates, (R)-o-chloromandelic acid was also produced in 89.5% yield and 98.6% ee.
Fenzi Cuihua published new progress about 13312-84-0. 13312-84-0 belongs to nitriles-buliding-blocks, auxiliary class Chloride,Nitrile,Benzene,Alcohol,Benzene Compounds, name is 2-(2-Chlorophenyl)-2-hydroxyacetonitrile, and the molecular formula is C12H23N3S, Quality Control of 13312-84-0.
Referemce:
https://en.wikipedia.org/wiki/Nitrile,
Nitriles – Chemistry LibreTexts