Van Genderen, H. published the artcileToxicological properties of the herbicide dichlobenil, 2,6-dichlorobenzonitrile, in warm-blooded animals, SDS of cas: 3336-34-3, the publication is Mededelingen Rijksfaculteit Landbouwwetenschappen, Gent (1966), 31(3), 1026-31, database is CAplus.
After oral administration of 100 mg. dichlobenil/kg. body weight, rabbits converted approx. 23% into 2,6-dichloro-3-hydroxybenzonitrile (I) and 2% into the 4-hydroxy analog (II). Rats excreted, resp., 22% and 9% of I and II. In mice the i.p. LD50 for I and II was 83 mg./kg. and 50 mg./kg., resp. The oral toxicity for mice and rabbits was very much lower. The effects of I and II were studied on the respiration of bakers’ yeast, on the ATPase induction in isolated mitochondria, on the contraction of isolated rat diaphragm, and on single beating rat heart cells in tissue culture. It was concluded that I and II, like other chlorophenols, were uncoupling agents of oxidative phosphorylation with approx. the same potency as 2,4-dinitrophenol. Liver function tests with dichlobenil showed different results for rats and rabbits. The effect on the liver of the rat seemed to be reversible, but, at a critical dose level for the rabbit, it was irreversible. It was suggested that in the rat liver cells the conjugation of the phenols to glucuronides and sulfates could keep pace with their formation.
Mededelingen Rijksfaculteit Landbouwwetenschappen, Gent published new progress about 3336-34-3. 3336-34-3 belongs to nitriles-buliding-blocks, auxiliary class Chloride,Nitrile,Benzene,Phenol, name is 2,6-Dichloro-3-hydroxybenzonitrile, and the molecular formula is C8H10S, SDS of cas: 3336-34-3.
Referemce:
https://en.wikipedia.org/wiki/Nitrile,
Nitriles – Chemistry LibreTexts