Bertini, Simone; Saccomanni, Giuseppe; DelCarlo, Sara; Digiacomo, Maria; Gargini, Claudia; Piano, Ilaria; Campisi, Giuseppe Matteo; Ghidoni, Riccardo; Macchia, Marco; Manera, Clementina published an article in 2017, the title of the article was Application of an improved HPLC-FL method to screen serine palmitoyl transferase inhibitors.Product Details of 34662-29-8 And the article contains the following content:
In this work, we reported the application and validation of an improved high-performance liquid chromatog. method coupled with a fluorimetric detector (HPLC-FL) to screen the activity of two heterocyclic derivatives reported as serine palmitoyl transferase (SPT) inhibitors. The anal. conditions were optimized in terms of the derivatization procedure, chromatog. condition, extraction procedure, and method validation according to EMEA guidelines. Once fully optimized, the method was applied to assess the SPT-inhibitory activity of the above-mentioned derivatives and of the reference inhibitor myriocin. The obtained results, expressed as a percentage of residual SPT activity, were compared to those obtained with the reference radio immune assay (RIA). The good correlation between the two types of assay demonstrated that the improved HPLC-FL method is suitable for a preliminary and rapid screening of potential SPT-inhibitors. The experimental process involved the reaction of 3-Chloro-4-nitrobenzonitrile(cas: 34662-29-8).Product Details of 34662-29-8
The Article related to serine palmitoyl transferase inhibitor hplcfl method, hplc, spt, enzymatic assay, screening, serine palmitoyl transferase, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Product Details of 34662-29-8
Referemce:
Nitrile – Wikipedia,
Nitriles – Chemistry LibreTexts