Wang, Zengmeng; Zhao, Jiulong; Wang, Long; Li, Chengcheng; Liu, Jianhui; Zhang, Lihua; Zhang, Yongyu published the artcile< A novel benthic phage infecting Shewanella with strong replication ability>, Product Details of C7H5N5O, the main research area is coastal sediment benthic phage shewanella phylogenetic analysis; Shewanella; benthic phage; coastal sediments; phage genome; phylogenetic analysis.
The coastal sediments were considered to contain diverse phages playing important roles in driving biogeochem. cycles based on genetic anal. However, till now, benthic phages in coastal sediments were very rarely isolated, which largely limits our understanding of their biol. characteristics. Here, we describe a novel lytic phage (named Shewanella phage S0112) isolated from the coastal sediments of the Yellow Sea infecting a sediment bacterium of the genus Shewanella. The phage has a very high replication capability, with the burst size of ca. 1170 phage particles per infected cell, which is 5-10 times higher than that of most phages isolated before. Meanwhile, the latent period of this phage is relatively longer, which might ensure adequate time for phage replication. The phage has a double-stranded DNA genome comprising 62,286 bp with 102 ORFs, ca. 60% of which are functionally unknown. The expression products of 16 ORF genes, mainly structural proteins, were identified by LC-MS/MS anal. Besides the general DNA metabolism and structure assembly genes in the phage genome, there is a cluster of auxiliary metabolic genes that may be involved in 7-cyano-7-deazaguanine (preQ0) biosynthesis. Meanwhile, a pyrophosphohydrolase (MazG) gene being considered as a regulator of programmed cell death or involving in host stringer responses is inserted in this gene cluster. Comparative genomic and phylogenetic anal. both revealed a great novelty of phage S0112. This study represents the first report of a benthic phage infecting Shewanella, which also sheds light on the phage-host interactions in coastal sediments.
Viruses published new progress about Coastal sediments. 69205-79-4 belongs to class nitriles-buliding-blocks, and the molecular formula is C7H5N5O, Product Details of C7H5N5O.
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