Kot, Witold; Olsen, Nikoline S.; Nielsen, Tue K.; Hutinet, Geoffrey; de Crecy-Lagard, Valerie; Cui, Liang; Dedon, Peter C.; Carstens, Alexander B.; Moineau, Sylvain; Swairjo, Manal A.; Hansen, Lars H. published the artcile< Detection of preQ0 deazaguanine modifications in bacteriophage CAjan DNA using Nanopore sequencing reveals same hypermodification at two distinct DNA motifs>, COA of Formula: C7H5N5O, the main research area is deazaguanine modification bacteriophage DNA nanopore sequencing.
In the constant evolutionary battle against mobile genetic elements (MGEs), bacteria have developed several defense mechanisms, some of which target the incoming, foreign nucleic acids e.g. restriction-modification (R-M) or CRISPR-Cas systems. Some of these MGEs, including bacteriophages, have in turn evolved different strategies to evade these hurdles. It was recently shown that the siphophage CAjan and 180 other viruses use 7-deazaguanine modifications in their DNA to evade bacterial R-M systems. Among others, phage CAjan genome contains a gene coding for a DNA-modifying homolog of a tRNA-deazapurine modification enzyme, together with four 7-cyano-7-deazaguanine synthesis genes. Using the CRISPR-Cas9 genome editing tool combined with the Nanopore Sequencing (ONT) we showed that the 7-deazaguanine modification in the CAjan genome is dependent on phage-encoded genes. The modification is also site-specific and is found mainly in two sep. DNA sequence contexts: GA and GGC. Homol. modeling of the modifying enzyme DpdA provides insight into its probable DNA binding surface and general mode of DNA recognition.
Nucleic Acids Research published new progress about Bacteriophage. 69205-79-4 belongs to class nitriles-buliding-blocks, and the molecular formula is C7H5N5O, COA of Formula: C7H5N5O.
Referemce:
Nitrile – Wikipedia,
Nitriles – Chemistry LibreTexts