Ferreira, Everton Geraldo Capote; Gomes, Douglas Fabiano; Delai, Caroline Vanzzo; Barreiros, Marco Antonio Bacellar; Grange, Luciana; Rodrigues, Elisete Pains; Henning, Liliane Marcia Mertz; Barcellos, Fernando Gomes; Hungria, Mariangela published the artcile< Revealing potential functions of hypothetical proteins induced by genistein in the symbiosis island of Bradyrhizobium japonicum commercial strain SEMIA 5079 (= CPAC 15)>, Synthetic Route of 69205-79-4, the main research area is Bradyrhizobium genistein cobalamin amino acids symbiosis Squalene hopene cyclase; Biological nitrogen fixation; Functional inference; Gene expression; Symbiosis.
Bradyrhizobium japonicum strain SEMIA 5079 (= CPAC 15) is a nitrogen-fixing symbiont of soybean broadly used in com. inoculants in Brazil. Its genome has about 50% of hypothetical (HP) protein-coding genes, many in the symbiosis island, raising questions about their putative role on the biol. nitrogen fixation (BNF) process. This study aimed to infer functional roles to 15 HP genes localized in the symbiosis island of SEMIA 5079, and to analyze their expression in the presence of a nod-gene inducer. A workflow of bioinformatics tools/databases was established and allowed the functional annotation of the HP genes. Most were enzymes, including transferases in the biosynthetic pathways of cobalamin, amino acids and secondary metabolites that may help in saprophytic ability and stress tolerance, and hydrolases, that may be important for competitiveness, plant infection, and stress tolerance. Putative roles for other enzymes and transporters identified are discussed. Some HP proteins were specific to the genus Bradyrhizobium, others to specific host legumes, and the anal. of orthologues helped to predict roles in BNF. All 15 HP genes were induced by genistein and high induction was confirmed in five of them, suggesting major roles in the BNF process.
BMC Microbiology published new progress about Acacia auriculiformis. 69205-79-4 belongs to class nitriles-buliding-blocks, and the molecular formula is C7H5N5O, Synthetic Route of 69205-79-4.
Referemce:
Nitrile – Wikipedia,
Nitriles – Chemistry LibreTexts