The three-dimensional configuration of the ester heterocycle is basically the same as that of the carbocycle. Compound: 5-Chloro-1-methyl-4-nitroimidazole(SMILESS: C1=NC(=C(Cl)[N]1C)[N+]([O-])=O,cas:4897-25-0) is researched.COA of Formula: C21H16N2. The article 《A validated UPLC method for the determination of process-related impurities in Azathioprine bulk drug》 in relation to this compound, is published in Analytical Methods. Let’s take a look at the latest research on this compound (cas:4897-25-0).
An UPLC method was developed and subsequently validated for the determination of Azathioprine and its process-related impurities. Separation was achieved with Acquity UPLC BEH C18, 100 × 2.1 mm, 1.7 μm column and trifluoroacetic acid (0.05% in water): acetonitrile as eluent in gradient mode. Flow rate was set at 0.35 mL min-1. UV detection was performed at 220 nm. The method was validated with respect to specificity, accuracy, precision, linearity, robustness, limit of quantification, and limit of detection. The accuracy of the method demonstrated at 3 levels in the range of 50-150% of the specification limit and the recovery of impurities were found to be in the range of 98 to 102%. The detection limits of the process related impurities ranged between 0.16 and 0.24 μg mL-1. The described method is simple, rapid, linear, precise, and robust. The method is useful during process development and quality control of bulk manufacturing
This compound(5-Chloro-1-methyl-4-nitroimidazole)Application In Synthesis of 5-Chloro-1-methyl-4-nitroimidazole was discussed at the molecular level, the effects of temperature and reaction time on the properties of the compound were discussed, and the optimum reaction conditions were selected.
Reference:
Nitrile – Wikipedia,
Nitriles – Chemistry LibreTexts