Reference of 5-Chloro-1-methyl-4-nitroimidazole. So far, in addition to halogen atoms, other non-metallic atoms can become part of the aromatic heterocycle, and the target ring system is still aromatic. Compound: 5-Chloro-1-methyl-4-nitroimidazole, is researched, Molecular C4H4ClN3O2, CAS is 4897-25-0, about Determination of two related substances in azathioprine tablets by HPLC.
An HPLC method was developed to determine 6-mercaptopurine and 5-chloro-1-methyl-4-nitroimidazole, which were related substances of azathioprine tablets. An ODS C18 column (250 mm × 4.6 mm, 5 μm) was used with the mobile phase of a mixture of sodium heptanesulfonate solution (sodium heptanesulfonate 1.336 g, dissolved in 850 mL water)-methanol (85:15, adjust the solution with 1 mol/L-1 hydrochloric acid to a pH of 3.5) at a flow rate of 1.0 mL/min-1. The detection wavelength was at 254 nm. For 6-mercaptopurine, the calibration curve was linear in the range of 4. 68 ± 10-4 to 0.014 mg/mL-1 with correlation coefficient 0.9997 and the regression equation being Y = 5400X-0.5302. The average recovery (n = 9) was 99.5%, and the detection limit was 1 ng . For 5-chloro-1-methyl-4-nitroimidazole, the calibration curve was also linear in the range of 5. 51 ± 10-4 to 0.0165 mg/mL-1 with correlation coefficient 0.9994 and the regression equation being Y = 6743X-1.396, the average recovery (n = 9) was 99.7%, and the detection limit was 2.5 ng. Sample solution and standard solution are all stable within 8 h. This method is specific, accurate, sensitive, and simple.
《Determination of two related substances in azathioprine tablets by HPLC》 provides a strategy for the preparation of materials with excellent comprehensive properties, which is conducive to broaden the application field of this compound(5-Chloro-1-methyl-4-nitroimidazole)Reference of 5-Chloro-1-methyl-4-nitroimidazole.
Reference:
Nitrile – Wikipedia,
Nitriles – Chemistry LibreTexts