In general, if the atoms that make up the ring contain heteroatoms, such rings become heterocycles, and organic compounds containing heterocycles are called heterocyclic compounds. An article called HPLC determination of the related substances in Azathioprine Tablets, published in 2013-02-28, which mentions a compound: 4897-25-0, Name is 5-Chloro-1-methyl-4-nitroimidazole, Molecular C4H4ClN3O2, COA of Formula: C4H4ClN3O2.
A HPLC method for determination of related substances in Azathioprine Tablets was established. The C18 column (200 mm×4.6 mm, 5 μm) was used. The mobile phase consisted of methanol and 0.05% of sodium acetate solution (18:82) at the flow rate of 1.2 mL·min-1, and the detection wavelength was set at 300 nm. The linear ranges of the two typical related substances mercaptopurine and 5-chloro-1-methyl-4-nitroimidazole in Azathioprine Tablets were all 0.125-1.80μg·mL-1 (r=0.9997 and 0.9999) with the detection limits of 0.787 ng and 0.933 ng, and the average recoveries were 100.3% and 100.0% with the RSD of 0.64% and 0.14%. The method proposed for determining the related substances of Azathioprine Tablets is simple, quick and the result is accurate and specific.
After consulting a lot of data, we found that this compound(4897-25-0)COA of Formula: C4H4ClN3O2 can be used in many types of reactions. And in most cases, this compound has more advantages.
Reference:
Nitrile – Wikipedia,
Nitriles – Chemistry LibreTexts