Synthetic Route of C4H4ClN3O2. The protonation of heteroatoms in aromatic heterocycles can be divided into two categories: lone pairs of electrons are in the aromatic ring conjugated system; and lone pairs of electrons do not participate. Compound: 5-Chloro-1-methyl-4-nitroimidazole, is researched, Molecular C4H4ClN3O2, CAS is 4897-25-0, about Azathioprine lymphocytotoxicity. Potentially lethal damage by its imidazole derivatives. Author is Sauer, H.; Hantke, U.; Wilmanns, W..
The immunosuppressive agent 6-(1-methyl-4-nitroimidazole-5-yl)thiopurine (azathioprine, AZA) is metabolized to the purine antagonist 6-mercaptopurine (6-MP) and to 5-substituted 1-methyl-4-nitro-5-thioimidazoles or aminoimidazoles. Besides the cytostatic (growth inhibition) and cytocidal (cell killing) effect of the anti-metabolite 6-MP, which can be antagonized by exogenous purine supplementation, AZA has an addnl. effect, which cannot be antagonized by purines. This effect is due to electron-affine imidazole derivatives, which are generated by a nucleophilic attack on the AZA mol. A synthetic imidazole derivative (1-methyl-4-nitro-5-chloroimidazole, MNCI) develops cytostatic and cytocidal effects in 2 different permanent human lymphoblastoid cultures (LS2 and CH4) with threshold concentrations of 8 × 10-5 and 6 × 10-5 M, resp. This imidazole derivative with the nitro and chloro group in ortho-position seems to be more reactive than other imidazole derivatives It interferes with the redox potential of the cells. It causes glutathione depletion and sublethal conditioning of the cells (reduced repair capacity = potentially lethal damage = PLD = chemosensitization).
There are many compounds similar to this compound(4897-25-0)Synthetic Route of C4H4ClN3O2. if you want to know more, you can check out my other articles. I hope it will help you，maybe you’ll find some useful information.
Reference:
Nitrile – Wikipedia,
Nitriles – Chemistry LibreTexts