Most of the natural products isolated at present are heterocyclic compounds, so heterocyclic compounds occupy an important position in the research of organic chemistry. A compound: 4897-25-0, is researched, SMILESS is C1=NC(=C(Cl)[N]1C)[N+]([O-])=O, Molecular C4H4ClN3O2Journal, Fenxi Ceshi Xuebao called Determination of ten nitroimidazole residues in royal jelly by high performance liquid chromatography tandem mass spectrometry, Author is Xie, Wen; Chen, Xiaomei; Ding, Huiying; Hou, Jianbo; Xi, Junyang; Qian, Yan, the main research direction is royal jelly high performance liquid chromatog tandem mass spectrometry; nitroimidazole residue.Electric Literature of C4H4ClN3O2.
Nitroimidazoles and their hydroxy metabolites are banned substances with antibiotic and anticoccidial activity. They are suspected to be carcinogenic and mutagenic. In this paper, a rapid, sensitive and selective method for the determination of ten nitroimidazole residues, including dimetridazole(DMZ), 2-hydroxymethyl-1-methyl-5-nitroimidazole(HMMNI) or dimetridazole-OH(DMZOH), metronidazole(MNZ), metronidazole-OH(MNZOH), ronidazole(RNZ), 5-nitrobenzimidazole(NBI), ipronidazole(IPZ), ipronidazole-OH(IPZOH), 2-methyl-5-nitroimidazole(MNI) and 5-chloro-1-methyl-4-nitroimidazole(CMNI) in royal jelly was established by liquid chromatog.-tandem mass spectrometry (LC-ESI MS/MS). Methanol was used to precipitate protein, the supernatant solution was extracted with Et acetate under weak basic condition and cleaned up with Oasis(HLB) and C18 columns. The quant. detection was performed by LC-MS/MS on multiple reaction monitoring(MRM) mode under pos.-ion electrospray ionization. The isotope internal standards(D3-DMZOH, D3-DMZ, D3-RNZ, D3-IPZOH, D3-IPZ) were added into the sample solutions, and isotope dilution internal standard method or external standard method was used for quantitation anal. The results showed that the calibration curves of ten nitroimidazole residues were linear in the range of 5.0-60μg/kg with correlation coefficients more than 0.999. The recoveries of ten nitroimidazole residues at the spiked levels of 10, 20 and 50μg/kg were between 70% and 105% with RSDs less than 12.7%, and the quantitation limits were all 10μg/kg. The method was specific and accurate, and was suitable for the determination and confirmation of nitroimidazole residues in royal jelly samples.
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Reference:
Nitrile – Wikipedia,
Nitriles – Chemistry LibreTexts