Related Products of 4640-67-9, In the next few decades, the world population will flourish. As the population grows rapidly and people all over the world use more and more resources, all industries must consider their environmental impact. 4640-67-9, name is 3-(4-Fluorophenyl)-3-oxopropanenitrile belongs to nitriles-buliding-blocks compound, it is a common compound, a new synthetic route is introduced below.
General procedure: LB medium supplemented with 30 mug/mL kanamycin was inoculated with a single colony of E. coli (containing the appropriate overexpressed gene) and shaken 15 h at 37 C. This preculture was diluted 1:100 into 30 mL of the same medium and shaken 2.5 h at 37 C with a stir rate of 220 rpm. Upon reaching an O.D.600 = 0.5 the cell culture was cooled to 20 C, and isopropylthio-beta-D-galactoside was added to a final concentration of 100 muM and shaken for 2 h. The beta-keto nitrile substrate was added to a final concentration of 2 mM using a concentrated acetonitrile solution. (Ethanol and methanol solutions were avoided due to an increase in alkylation). This reaction was allowed to shake at 220 rpm for 12 h. The reaction was then gently extracted with methylene chloride (2 mL) and analyzed by chiral GCMS.
The synthetic route of 3-(4-Fluorophenyl)-3-oxopropanenitrile has been constantly updated, and we look forward to future research findings.
Reference:
Article; Nowill, Randall W.; Patel, Trisha J.; Beasley, David L.; Alvarez, Jose A.; Jackson III, Elizah; Hizer, Todd J.; Ghiviriga, Ion; Mateer, Scott C.; Feske, Brent D.; Tetrahedron Letters; vol. 52; 19; (2011); p. 2440 – 2442;,
Nitrile – Wikipedia,
Nitriles – Chemistry LibreTexts